Search results for "methods [Biological Assay]"
showing 10 items of 243 documents
Structure and determinants of production in Textile-Clothing-Leather-Skins (TCLS) craft industry in Benin: a study based on investigations of the val…
2014
Lieu et date de la conférence modifiées. Prétoria 31Juillet au 2 Août 2014 (Au lieu de Kigali -Rwanda); International audience; Sustainable economic growth in Benin requires a better understand of the informalsector, which contributes to two-thirds of GDP. Particularly, craft industry and TCLSsubsector is one of informal activity sector to be handled. The objectives of this work wereto identify the structure and factors that determine the production in TCLS craft industry.This study was based on a survey carried out in February 2011 on value creationdata in craft industry of TCLS in Benin. Lack of data induced by informal activities wascircumvented using an imputation method. A Multiple Cor…
Brooches in Motion - tracing shape evolution in La Tène brooches by geometric morphometrics
2018
International audience
Some analytical considerations on two-scale relations
1994
Scaling functions that generate a multiresolution analysis (MRA) satisfy, among other conditions, the so-called «two-scale relation» (TSR). In this paper we discuss a number of properties that follow from the TSR alone, independently of any MRA: position of zeros (mainly for continuous scaling functions), existence theorems (using fixed point and eigenvalue arguments) and orthogonality relation between integer translates. © 1994 Società Italiana di Fisica.
Time characteristics of Lévy flights in a steep potential well
2013
Using the method previously developed for ordinary Brownian diffusion, we derive a new formula to calculate the correlation time of stationary Lévy flights in a steep potential well. For the symmetric quartic potential, we obtain the exact expression of the correlation time of steady-state Lévy flights with index α = 1. The correlation time of stationary Lévy flights decreases with an increasing noise intensity and steepness of potential well.
Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…
2019
Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
2009
We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flanking a given splice junction allows to specifically quantify, within the cell, the RNA isoform generating such splice junction by FRET measure. As a proof of concept we analyzed two alternative splicing events originating from lymphocyte antigen 6 (LY6) complex, locus G5B (LY6G5B) pre-mRNA. These are characterized by the removal of the first intron (Fully Spliced Isoform, FSI) or by retention of suc…
Real-time Fluorescence Measurement of Enterovirus Uncoating
2019
Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative staining, cryo electron microscopy, X-ray crystallography or gradient separation (reviewed in Tuthill et al., 2010, Myllynen et al., 2016, Ruokolainen et al., 2019). However, monitoring of uncoating has been limited by the lack of methods detecting dynamic changes of the virions. Here, we present a real-time fluorescence based protocol, which detects the viral …
An LMI approach to vibration control of base-isolated building structures with delayed measurements
2010
In this article, we address a convex optimisation approach to the problem of state-feedback H∞ control design for vibration reduction of base-isolated building structures with delayed measurements, where the delays are time-varying and bounded. An appropriate Lyapunov-Krasovskii functional and some free-weighting matrices are utilised to establish some delay-range-dependent sufficient conditions for the design of desired controllers in terms of linear matrix inequalities. The controller, which guarantees asymptotic stability and an H∞ performance, simultaneously, for the closed-loop system of the structure, is then developed. The performance of the controller is evaluated by means of simula…
Descriptors of Posidonia oceanica meadows: Use and application
2005
This work benefited partly from the financial support of the European program ΓNTERREG IIIA Corsica, Sardinia. Tuscany
The Making of the New European Wind Atlas - Part 2: production and evaluation
2020
This is the second of two papers that document the creation of the New European Wind Atlas (NEWA). In Part 1, we described the sensitivity experiments and accompanying evaluation done to arrive at the final mesoscale model setup used to produce the mesoscale wind atlas. In this paper, Part 2, we document how we made the final wind atlas product, covering both the production of the mesoscale climatology generated with the Weather Research and Forecasting (WRF) model and the microscale climatology generated with the Wind Atlas Analysis and Applications Program (WAsP). The paper includes a detailed description of the technical and practical aspects that went into running the mesoscale simulati…